What is DNA Polymerase Proofreading?
DNA polymerase proofreading is a mechanism that explains why DNA replication is not perfect.
The enzyme DNA polymerase will read both strands of the parent DNA, but it will make mistakes and create mutations in the daughter strand. These mutations can lead to cancerous cells if left unrepaired. Proofreading enzymes then correct any mistakes that the DNA polymerase made while copying.
How DNA Polymerase Proofreading Works
DNA polymerase is an enzyme that catalyses the formation of a new DNA strand. The enzyme is also known as DNA polymerase I, and it can only repair single base mutation errors.
The way it works is that the enzyme reads one strand of DNA and when it finds a mismatch, it moves to the other end of the mismatched bases and inserts a complementary nucleotide. It then checks whether or not the insertion was correct by looking for any mismatched base on the other strand of DNA.
DNA proofreading can be done without any outside help because there are an additional four enzymes within this process. These four enzymes are adenosine deaminase (ADA), ribonuclease H (RNase H), apurinic/apyrimidinic endonuclease (APE)
Why You Need to Speed Up Your DNA Polymerase Proofreading
DNA polymerase proofreading is a process that corrects unpaired bases in DNA.
In order for the cells to divide, the DNA has to be properly copied. However, when a mistake is made in the copying process, it can cause an entire row of bases to be deleted. This can lead to all sorts of problems with people’s health such as cancer because the mutated genes are passed on.
To avoid these types of mistakes from happening, you need to speed up your DNA polymerase proofreading process which means the enzymes will make less mistakes when it comes time for replication.
DNA polymerase proofreading is an important step that helps avoid mutations and cell death by fixing errors that occur during replication. In order for this enzyme to work at its best, it needs to be running as quickly as
Methods for Faster DNA Polymerase Proofreading
One way to increase DNA polymerase proofreading is to use a different DNA polymerase. The other is to use DNA polymerase inhibitors.
The two ways that can be used to increase the ability of DNA polymerases in proofreading are by using a different DNA polymerase and by using inhibitors for the enzyme.
The first method is accomplished by using a different enzyme that has an improved ability to proofread mismatched bases. A disadvantage of this method would be that it may not work with all types of organisms because some organisms have only one type of polymerase and there is no variation in these enzymes. The second method uses inhibitors for enzymes, which decreases the activity of the enzyme and increases its efficiency when it comes to accurately reading sequences.